[Evaluation of CK-MB determination technique by immuno-enzymolfluorometry with Vidas (bioMérieux)]

The aim of this study was to evaluate a new automated fluorimetric enzyme immunoassay for CK-MB using the Vidas bioMérieux. The results for sera from 222 patients were compared with those obtained using two other methods of CK-MB determination in mass units (Stratus II Dade, Magic Lite Analyzer II Ciba Corning). Satisfactory results were obtained for within-run precision (CV < 9%) and between-run precision (CV < 10%) with medium (20 micrograms.L-1) and high (100 micrograms.L-1) concentrations of control sera. The method was linear in the range 1.7 micrograms.L-1 to 235 micrograms.L-1. Comparison with the two other techniques led to the following regression equations: y (Vidas) = 0.512 + 1.319 x (Stratus) [r = 0.991] and y (Vidas) = -0.15 + 0.795 x (Magic Lite) [r = 0.958]. The results obtained are highly correlated (p < 0.0001). The reference values were established on sera from healthy donors (n = 77). The Vidas technique gave analytical and clinical results similar to those of the other immunoenzymatic methods of CK-MB determination.