Related Experiment Videos
Development of a gas-phase oxygen biosensor using a blue copper-containing oxidase
A E Gardiol1, R J Hernandez, B Reinhammar
1School of Packaging, Michigan State University, East Lansing 48824-1223, USA.
Enzyme and Microbial Technology
|April 1, 1996
Summary
A novel gas-phase oxygen biosensor was developed using blue copper oxidases. This enzyme-based sensor detects oxygen by monitoring color changes, offering a stable method for monitoring oxygen levels.
Area of Science:
- Biotechnology
- Enzyme-based biosensors
- Oxygen detection
Background:
- Blue copper oxidases, like laccase, possess a blue chromophore (Type 1 Cu+2).
- This chromophore is reduced and decolorized by substrates, then reoxidized and recovers color with molecular oxygen.
Purpose of the Study:
- To develop a gas-phase oxygen biosensor utilizing blue copper-containing oxidases.
- To assess the operational stability and response time of the developed biosensor.
Main Methods:
- Utilized Rhus vernicifera laccase and ascorbate as substrate within low-density polyethylene pouches under nitrogen.
- Measured gas-phase oxygen by monitoring the rate of blue color reappearance spectrophotometrically at 610 nm.
- Assessed operational stability at 5°C with varying oxygen/nitrogen mixtures.
Main Results:
- The biosensor demonstrated the ability to detect a wide range of gas-phase oxygen concentrations.
- Optimized conditions (5°C, high water activity) determined the biosensor response time.
- The biosensor exhibited adequate activity and stability for oxygen measurement.
Conclusions:
- A functional gas-phase oxygen biosensor based on blue copper oxidase was successfully developed.
- The biosensor is suitable for measuring oxygen concentrations at 5°C and high water activity.
- Potential applications include monitoring headspace oxygen in packaged products to ensure quality and safety.