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Related Experiment Videos

Adenosine triphosphate levels in human plasma

L M Ryan1, J W Rachow, B A McCarty

  • 1Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA.

The Journal of Rheumatology
|February 1, 1996
PubMed
Summary
This summary is machine-generated.

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Plasma adenosine triphosphate (ATP) levels drop significantly after venipuncture, independent of food intake or exercise. Diurnal variations in ATP suggest it is not solely from platelet release.

Area of Science:

  • Biochemistry
  • Physiology

Background:

  • Extracellular adenosine triphosphate (ATP) is implicated in various physiological processes.
  • Synovial fluid ATP is a potential factor in articular tissue function.
  • Understanding plasma ATP dynamics is crucial for interpreting its role in joint health.

Purpose of the Study:

  • Quantify extracellular ATP in human platelet-poor plasma.
  • Investigate plasma ATP as a source for synovial fluid ATP.
  • Identify factors influencing plasma ATP levels.

Main Methods:

  • Adenosine triphosphate (ATP) measured using a luciferase assay.
  • Platelet beta thromboglobulin assessed via radioimmunoassay.
  • Serial venipuncture and indwelling venous catheters used to collect samples.

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Main Results:

  • Plasma ATP showed a consistent 58% decrease 15 minutes post-venipuncture.
  • This ATP drop was unaffected by fasting, feeding, or indomethacin.
  • Forearm exercise did not alter plasma ATP levels; diurnal variation with troughs at noon and night was observed.

Conclusions:

  • Venipuncture-induced plasma ATP fall and diurnal variation indicate ATP independent of platelet release.
  • Plasma ATP alone is insufficient to account for extracellular ATP in articular tissues.
  • Further research is needed to elucidate the precise origins and functions of plasma ATP.