Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Programmed cell death in bacteria: translational repression by mRNA end-pairing

T Franch1, K Gerdes

  • 1Department of Molecular Biology, Odense University, Denmark.

Molecular Microbiology
|September 1, 1996
PubMed
Summary

Plasmid maintenance systems use stable killer mRNAs (hok/pnd) and unstable antisense RNAs. Fold-back inhibition (fbi) elements sequester translational activators (tac) in inert mRNAs until 3' processing removes fbi, enabling translation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Landscape review about the decision to euthanize a compromised pig.

Porcine health management·2024
Same author

Expression of heat-shock protein 90 in glucocorticoid-sensitive and -resistant childhood acute lymphoblastic leukaemia.

Leukemia·2003
Same author

A pH-jump approach for investigating secondary structure refolding kinetics in RNA.

Nucleic acids research·2002
Same author

Bacterial toxin RelE induces apoptosis in human cells.

FEBS letters·2002
Same author

The double par locus of virulence factor pB171: DNA segregation is correlated with oscillation of ParA.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

RelE, a global inhibitor of translation, is activated during nutritional stress.

Proceedings of the National Academy of Sciences of the United States of America·2001

Area of Science:

  • Molecular Biology
  • Genetics
  • Microbiology

Background:

  • Plasmid maintenance relies on toxic gene expression systems like hok/sok and pnd.
  • These systems utilize stable killer mRNAs and unstable antisense RNAs for regulation.
  • Previous work identified negative translational control elements in hok and pnd mRNAs.

Purpose of the Study:

  • To elucidate the regulatory mechanisms of hok and pnd mRNA translation.
  • To investigate the role of 3' fold-back inhibition (fbi) elements in mRNA regulation.
  • To identify and characterize translational activators (tac) at the 5' end of these mRNAs.

Main Methods:

  • Genetic complementation assays to study mRNA-protein interactions.
  • Mutational analyses to investigate the function of fbi and tac elements.

Related Experiment Videos

  • 3' exonucleolytical processing assays to determine processing rates and effects.

    • Main Results:

    • 3' fbi elements pair with 5' mRNA ends, maintaining an inert configuration and low processing rate.
    • Translational activators (tac) at the 5' end are sequestered by fbi elements in full-length mRNAs.
    • 3' processing removes fbi elements, triggering 5' end refolding and enabling translation by unmasking tac elements.

    Conclusions:

    • The hok and pnd mRNA regulatory system involves a novel mechanism of translational control.
    • 3' processing is crucial for activating translation by removing inhibitory elements and facilitating mRNA refolding.
    • This intricate regulation ensures efficient plasmid maintenance through controlled expression of toxic genes.