Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lactacystin increases LDL receptor level on HepG2 cells

H Miura1, H Tomoda, K Miura

  • 1Research Center for Biological Function, Kitasato Institute, Tokyo, Japan.

Biochemical and Biophysical Research Communications
|October 23, 1996
PubMed
Summary

The proteasome down-regulates low-density lipoprotein (LDL) receptor expression. Inhibiting the proteasome with lactacystin increased LDL receptor numbers in HepG2 cells, revealing a key regulatory role.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[A detection method for recombinant DNA from genetically modified maize CBH351].

Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·2001
Same author

[Pachymeningitis with autonomic dysfunctions: a case report].

No to shinkei = Brain and nerve·2001
Same author

[Evaluation of dysmorphic red cells in the urinary sediment].

Rinsho byori. The Japanese journal of clinical pathology·2001
Same author

Effect of femoral and tibial component position on patellar tracking following total knee arthroplasty: 10-year follow-up of Miller-Galante I knees.

The American journal of knee surgery·2001
Same author

Comparison of postoperative early active mobilization and immobilization in vivo utilising a four-strand flexor tendon repair.

Journal of hand surgery (Edinburgh, Scotland)·2001
Same author

Shh and Ptc are associated with taste bud maintenance in the adult mouse.

Mechanisms of development·2001

Area of Science:

  • Molecular biology
  • Cell biology
  • Biochemistry

Background:

  • Low-density lipoprotein (LDL) receptor expression is crucial for cholesterol homeostasis.
  • Proteolysis of transcription factors, like sterol regulatory element binding proteins (SREBPs), regulates LDL receptor levels.
  • The role of the proteasome in this regulatory pathway requires further investigation.

Purpose of the Study:

  • To investigate the involvement of the proteasome in the regulation of LDL receptor expression.
  • To determine the effect of proteasome inhibition on LDL receptor levels in cultured cells.

Main Methods:

  • Utilized HepG2 cells, a human hepatoma cell line.
  • Administered lactacystin, a specific proteasome inhibitor.
  • Quantified changes in LDL receptor expression.

Related Experiment Videos

Main Results:

  • Lactacystin treatment led to a significant increase in the number of LDL receptors on HepG2 cells.
  • This demonstrates that proteasome activity normally limits LDL receptor expression.

Conclusions:

  • The proteasome plays a critical down-regulatory role in controlling LDL receptor expression.
  • Targeting the proteasome may offer a novel strategy for modulating LDL metabolism.