Type C particles in culture of human glioblastoma cells

A long-term cell culture of human glioblastoma was investigated microscopically, virologically, and biochemically. Reverse transcriptase activity was detected in cultured human glioblastoma cells. 3H uridine was incorporated into particles of buoyant density at 1.07 g/ml (Ficoll) which is equal to that of Oncorna virus particles, but 3H thymidine was not incorporated at all. Furthermore, reverse transcriptase activity was also demonstrated with the particles, suggesting that the cultured human glioblastoma cells were producing type C Oncorna virus. Ultrastructural observations of cell culture of glioblastoma showed type C virus particles in cisternae and culture medium. Budding of the virus was also seen on the surface of the cell. The mean diameter of the particles was approximately 100 nm. Ca. 1.1 nm of spikes protruded from the envelope. Both types of virions were observed, i.e. the doughnut-shaped type form and the solid circular form.