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Bacterial translocation after mesenteric ligation in dogs
C Bibbo1, A J Petschenik, M T Reddell
1Department of Surgery, Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA.
Summary
Bacterial translocation of Escherichia coli did not impact the viability of ischemic small bowel in dogs. Hemodynamic changes associated with sepsis also did not correlate with survival outcomes in this study.
Area of Science:
- Gastroenterology
- Surgical Research
- Microbiology
Background:
- Ischemic bowel conditions can lead to bacterial translocation.
- The impact of Escherichia coli translocation on intestinal viability remains unclear.
Purpose of the Study:
- To investigate the relationship between Escherichia coli translocation and the viability of ischemic small bowel in a canine model.
- To determine if bacterial translocation or altered hemodynamic parameters correlate with survival after intestinal ischemia.
Main Methods:
- Twenty beagles were gavaged with 14C-labeled E. coli at 3 or 24 hours before ileum ligation.
- Bacterial cultures and hemodynamic parameters were assessed before and after ischemia and resection.
- Mesenteric lymph node biopsies and bowel wall samples were analyzed for E. coli.
Main Results:
- E. coli translocation into mesenteric lymph nodes was higher with 3-hour pre-gavage interval.
- Positive E. coli cultures in mesenteric venous blood increased post-ligation.
- No correlation was found between E. coli translocation (blood, peritoneal fluid, lymph nodes, bowel wall) and survival.
- Altered hemodynamic parameters did not correlate with survival.
Conclusions:
- Bacterial translocation of E. coli does not affect the viability of ischemic small bowel in this canine model.
- Survival in cases of intestinal ischemia is not influenced by the degree of E. coli translocation or specific hemodynamic changes.