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Mutations in sheep microsatellites

A M Crawford, R P Cuthbertson

    Genome Research
    |September 1, 1996
    PubMed
    Summary
    This summary is machine-generated.

    Researchers studied sheep genome microsatellites, observing five spontaneous mutations. This established a mutation rate of 1.1 x 10(-4) per gamete per locus, crucial for sheep genetic mapping.

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    TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

    Area of Science:

    • Genomics
    • Molecular Biology
    • Animal Genetics

    Background:

    • Microsatellites are vital genetic markers for genome mapping.
    • Understanding microsatellite mutation rates is essential for accurate genetic analysis in livestock.

    Purpose of the Study:

    • To determine the spontaneous mutation rate of microsatellites in sheep.
    • To identify and characterize mutations during sheep genome linkage map construction.

    Main Methods:

    • Genotyping of 46,225 parent-offspring microsatellite allele transfers in the AgResearch International Mapping Flock.
    • Rechecking potential mutations with new DNA samples.
    • Utilizing DNA from untransformed lymphocytes.

    Main Results:

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  • Five bona fide microsatellite mutations were identified.
  • The observed spontaneous mutation rate was 1.1 +/- 0.5 x 10(-4) mutations/gamete/locus.
  • All mutations involved a 2-bp insertion in heterozygous individuals.

    • Conclusions:

    • The study provides a precise spontaneous mutation rate for sheep microsatellites.
    • Mutation timing varied, with some occurring during meiosis or male germ cell mitosis, and others during early female germ cell mitosis.
    • Findings contribute to refining sheep genome mapping and genetic studies.