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Related Experiment Videos

Eicosanoids modulate hyperpnea-induced bronchoconstriction in canine peripheral airways

C Omori1, P Tagari, A N Freed

  • 1Department of Environmental Health Sciences, Johns Hopkins University, Baltimore, Maryland 21205, USA.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|September 1, 1996
PubMed
Summary

Leukotrienes (LTs) play a key role in dry air-induced bronchoconstriction (AIB). Inhibiting leukotriene biosynthesis significantly reduces AIB in canine airways.

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Area of Science:

  • Respiratory Physiology
  • Pharmacology
  • Immunology

Background:

  • Dry air challenges can induce bronchoconstriction (AIB), a phenomenon not fully understood.
  • Leukotrienes (LTs) are implicated in airway inflammation and smooth muscle contraction.

Purpose of the Study:

  • To investigate the specific role of leukotrienes in the development of dry air-induced bronchoconstriction (AIB) in canine peripheral airways.
  • To assess the impact of inhibiting leukotriene biosynthesis on AIB.

Main Methods:

  • Airway reactivity to exogenous LTD4 was measured.
  • AIB was induced by a dry air challenge (DAC) in canine airways.
  • The effects of a peptidoleukotriene biosynthesis inhibitor (MK-0591) on AIB and airway wall temperature were evaluated.

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  • Eicosanoid levels in bronchoalveolar lavage fluid were quantified.

    • Main Results:

    • Exogenous LTD4 significantly increased peripheral airway resistance.
    • MK-0591 treatment attenuated AIB by approximately 65% without affecting airway wall temperature.
    • MK-0591 specifically reduced LT levels in bronchoalveolar lavage fluid.
    • AIB correlated with LTC4, LTD4, and LTE4 generation before MK-0591 treatment.

    Conclusions:

    • Hyperpnea with dry air stimulates local leukotriene production in canine bronchi.
    • Leukotrienes play a central role in modulating dry air-induced bronchoconstriction.
    • Prostaglandins also contribute to the modulation of AIB, with different correlations observed after LT inhibition.