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Related Experiment Videos

Validation procedures for the Hamilton Thorne Integrated Visual Optical System sperm and cell analyzer

D H Douglas-Hamilton1

  • 1Hamilton Thorne Research, Beverly, MA 01915, USA.

Quality Assurance (San Diego, Calif.)
|December 1, 1995
PubMed
Summary

The Integrated Visual Optical System (IVOS) analyzer accurately measures sperm parameters in lab animals for reproductive toxicology. Its digital system ensures reliable, validated results for on-site analysis, meeting Good Laboratory Practices.

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Area of Science:

  • Reproductive Toxicology
  • Sperm Analysis
  • Laboratory Animal Science

Background:

  • Sperm analysis is crucial in reproductive toxicology studies.
  • Accurate measurement of sperm motility and concentration is essential for reliable results.
  • Existing methods require validation for on-site application and Good Laboratory Practices (GLP) compliance.

Purpose of the Study:

  • To evaluate the Hamilton Thorne Integrated Visual Optical System (IVOS) analyzer for sperm analysis in laboratory animals.
  • To assess the accuracy and reliability of the IVOS system for measuring sperm motility and concentration.
  • To determine the system's suitability for on-site validation according to GLP standards.

Main Methods:

  • The IVOS system, combining optical and computer analysis, was used to assess sperm motility, progressive motility, velocity, and concentration.

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  • Digital encoding allowed for image storage and absolute replay fidelity for reexamination.
  • Validation involved a NIST-certified scale for length standards and manual confirmation of parameters using Cartesian coordinates.
  • Sperm concentration was determined using a DNA stain to differentiate sperm from somatic cells.

    • Main Results:

    • The IVOS system demonstrated high agreement (within 0.2%) between manual and computed sperm motion parameters.
    • Sperm concentration measurements by IVOS in rat testis and epididymis homogenates were within 5% of manual hemacytometer counts.
    • The system facilitates on-site validation through digital image storage and replay capabilities.

    Conclusions:

    • The Hamilton Thorne IVOS analyzer is a validated tool for accurate sperm analysis in laboratory animals.
    • The system's features support on-site validation and compliance with Good Laboratory Practices.
    • IVOS provides reliable measurements of sperm motility and concentration, crucial for reproductive toxicology research.