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Monoclonal antibody based differentiation of Coxiella burnetii isolates
Z Sekeyová1, D Thiele, H Krauss
1Institut für Hygiene und Infektionskrankheiten, Tiere, Justus-Liebig-Universität, Giessen, FRG.
Abstract:
Isolates of Coxiella burnetii from different geographic regions in Europe, USA, Japan and Africa were compared in their binding properties to the monoclonal antibody (MoAb) 1/4/H directed against the lipopolysaccharide (LPS) of C. burnetii strain Priscilla. Immunoblot analysis and enzyme-linked immunosorbent assay (ELISA) revealed different binding patterns of C. burnetii isolates under study. Most of the isolates tested did react with MoAb 1/4/H. Only four of 20 groups of isolates and one isolate of an otherwise positively reacting group did not react with MoAb 1/4/H. The results indicate a significant variation of LPS structure of the C. burnetii isolates studied.