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Related Experiment Videos

Positional information and opsin identity in retinal cones

K C Wikler1, A Szel, A L Jacobsen

  • 1Section of Neurobiology, Yale School of Medicine, New Haven, Connecticut 06510, USA.

The Journal of Comparative Neurology
|October 7, 1996
PubMed
Summary

Environmental cues do not regulate opsin expression in mouse cone photoreceptors postnatally. Positional information and opsin identity are determined before birth, challenging the hypothesis of aberrant opsin induction.

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Area of Science:

  • Developmental biology
  • Neuroscience
  • Ophthalmology

Background:

  • Cone photoreceptors are crucial for vision, expressing specific opsins like middle wavelength-sensitive (MWS) and short wavelength-sensitive (SWS).
  • The spatial segregation of MWS and SWS cones in the mouse retina suggests potential regulation by environmental cues.

Purpose of the Study:

  • To investigate whether local environmental cues regulate MWS and SWS opsin expression in neonatal mouse cone photoreceptors.
  • To determine if immature cones can be induced to express aberrant opsins by postnatal environmental factors.

Main Methods:

  • Utilized an organotypic culture system to examine neonatal mouse retinal development.
  • Harvested retinal explants from mice aged postnatal day 0 (P0) to P18, culturing them for up to 4 weeks.

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  • Employed opsin-specific antibodies (OS-2, JH455, JH492) for immunodetection and performed coculture experiments.

    • Main Results:

    • SWS opsin expression was observed in vitro within 5 days in newborn retinal cultures, mirroring in vivo timelines.
    • MWS opsin expression was only detected in explants from P3 or older mice, indicating a delayed and specific developmental pattern.
    • Coculturing dorsal and ventral retinal explants did not alter cone field emergence or opsin expression timing/regionality.

    Conclusions:

    • Positional information and opsin identity in mouse cone photoreceptors are established prior to birth.
    • Postnatal environmental cues do not induce immature cones to express aberrant opsins, refuting the initial hypothesis.