Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PCR analysis of polymorphisms at the D13S25 locus

R E Ibbotson1, R M Chapman, M M Corcoran

  • 1Molecular Biology, Pathology Department, Bournemouth General Hospital, UK.

Leukemia
|November 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-risk molecular features may eclipse genomic complexity in predicting chronic lymphocytic leukemia outcomes; UK clinical trial insights.

Leukemia·2026
Same author

Non-coding NOTCH1 mutations in chronic lymphocytic leukemia; their clinical impact in the UK CLL4 trial.

Leukemia·2016
Same author

Genomic disruption of the histone methyltransferase SETD2 in chronic lymphocytic leukaemia.

Leukemia·2016
Same author

Longitudinal copy number, whole exome and targeted deep sequencing of 'good risk' IGHV-mutated CLL patients with progressive disease.

Leukemia·2016
Same author

Telomere length predicts progression and overall survival in chronic lymphocytic leukemia: data from the UK LRF CLL4 trial.

Leukemia·2015
Same author

Quantification of subclonal distributions of recurrent genomic aberrations in paired pre-treatment and relapse samples from patients with B-cell chronic lymphocytic leukemia.

Leukemia·2012

Researchers identified a polymorphic marker near a potential tumor suppressor gene linked to B-cell chronic lymphocytic leukemia (B-CLL). PCR amplification of this region significantly improved diagnostic informativity to 80%.

Area of Science:

  • Genetics
  • Oncology
  • Molecular Biology

Background:

  • The D13S25 locus is implicated in the pathogenesis of B-cell chronic lymphocytic leukemia (B-CLL).
  • Loss of heterozygosity (LOH) and bi-allelic loss at this locus suggest the presence of a tumor suppressor gene.

Purpose of the Study:

  • To identify and characterize polymorphic markers at the D13S25 locus.
  • To enhance the informativity of genetic markers for B-CLL research.

Main Methods:

  • Detection of LOH and bi-allelic loss using the pH2-42 probe.
  • Sequencing of adjacent clones to identify SspI polymorphism.
  • Identification of a polymorphic (TA)n repeat.
  • Polymerase Chain Reaction (PCR) amplification of the region containing both markers.

Related Experiment Videos

Main Results:

  • A novel polymorphic (TA)n repeat was identified adjacent to the SspI polymorphism.
  • PCR amplification encompassing both markers increased the informativity of the D13S25 locus to 80%.

Conclusions:

  • The identified polymorphic markers provide a more informative tool for investigating the D13S25 locus in B-CLL.
  • Enhanced genetic marker informativity aids in understanding the role of tumor suppressor genes in B-CLL pathogenesis.