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Published on: December 27, 2014
Monocyte and granulocyte CD11b/CD18, CD62L expression and sICAM-1 concentration in the interdialytic period
P Thylén1, E Fernvik, J Lundahl
1Department of Internal Medicine, Karolinska Hospital, Stockholm, Sweden.
Abstract:
We studied cell surface modulation of CD11b/CD18 and CD62L on monocytes and granulocytes, sICAM-1 concentrations and the responsiveness of cells to exogenous fMLP in patients in the intra- (0-4 h Cuprophan dialysis) and interdialytic period (5-28 h) and in healthy subjects (0-24 h). The high CD11b/CD18, low CD62L granulocyte phenotype occurred rapidly during dialysis. By contrast, CD62L increased on the subpopulation of monocytes in circulation initially during dialysis and CD11b/CD18 was mobilized much slower. In the interdialytic period, the CD62L/(CD11b/CD18) ratio was reduced up to 12 h after start of treatment on both monocytes and granulocytes. This ratio was significantly lower than in healthy subjects up to 8 h after start of treatment. The responsiveness of granulocytes to exogenous fMLP, in terms of CD11b/CD18 mobilization, was significantly reduced in patients during and after hemodialysis as compared to that on granulocytes obtained from healthy controls. Monocytes were more refractory to fMLP up to 4 h after dialysis. sICAM-1 was significantly increased in patients before dialysis as compared to controls and remained elevated and fairly stable throughout treatment and in the interdialytic period. The variation in the expression of adhesion molecules on monocytes and on granulocytes in the interdialytic period was not related to the presence of activating serum factors remaining in the circulation after treatment. Our findings emphasize the importance of including the interdialytic period in the evaluation of dialysis membrane biocompatibility, especially when effects on monocytes are of interest.
Insights
Hemodialysis alters immune cell adhesion molecules (CD11b/CD18, CD62L) and reduces responsiveness, persisting into the interdialytic period. Evaluating dialysis membrane biocompatibility requires assessing this extended impact, particularly on monocytes.
Area of Science:
- Immunology
- Nephrology
- Biomaterials Science
Background:
- Hemodialysis involves blood contact with artificial membranes, potentially activating immune cells.
- Understanding immune cell modulation during and after dialysis is crucial for assessing membrane biocompatibility.
Purpose of the Study:
- To investigate the dynamic changes in monocyte and granulocyte adhesion molecules (CD11b/CD18, CD62L) and their responsiveness to fMLP.
- To evaluate soluble ICAM-1 (sICAM-1) levels and cell behavior during and after hemodialysis.
- To assess the influence of the interdialytic period on these immune parameters and relate findings to dialysis membrane biocompatibility.
Main Methods:
- Flow cytometry was used to measure cell surface expression of CD11b/CD18 and CD62L on monocytes and granulocytes.
- Quantification of soluble ICAM-1 (sICAM-1) concentrations in patient plasma.
- Assessment of cellular responsiveness to exogenous formyl-methionyl-leucyl-phenylalanine (fMLP) stimulation.
- Comparison of immune cell parameters between hemodialysis patients (intra- and interdialytic periods) and healthy subjects.
Main Results:
- A rapid increase in CD11b/CD18 and decrease in CD62L on granulocytes occurred during dialysis.
- Monocytes showed delayed CD11b/CD18 mobilization and initial CD62L increase during dialysis.
- The CD62L/CD11b/CD18 ratio remained altered in both cell types during the interdialytic period.
- Granulocyte and monocyte responsiveness to fMLP was significantly reduced post-hemodialysis.
- sICAM-1 levels were elevated in patients before, during, and after dialysis compared to controls.
Conclusions:
- Hemodialysis induces significant, prolonged alterations in immune cell adhesion molecule expression and function.
- The interdialytic period is critical for evaluating the full impact of dialysis membrane biocompatibility on immune cells, especially monocytes.
- Reduced cellular responsiveness suggests impaired immune surveillance post-dialysis, necessitating further investigation.
