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Microbial contamination in an in vitro fertilization-embryo transfer system
E Cottell1, J McMorrow, B Lennon
1Academic Department of Obstetrics and Gynaecology, Royal College of Surgeons in Ireland, Dublin.
Fertility and Sterility
|November 1, 1996
Summary
Microbial contamination in In Vitro Fertilization-Embryo Transfer (IVF-ET) cycles can originate from seminal fluid and oocytes. Penicillin and streptomycin-rich media effectively control these microbes without impacting IVF success rates.
Area of Science:
- Reproductive medicine
- Microbiology
- In Vitro Fertilization
Background:
- Microbial contamination poses a risk in assisted reproductive technologies like IVF-ET.
- Identifying sources and transmission routes is crucial for maintaining successful outcomes.
Purpose of the Study:
- To investigate the origins and spread of microorganisms within the IVF-ET process.
- To evaluate the effectiveness of antibiotic-supplemented culture media in controlling contamination.
Main Methods:
- A prospective study was conducted in an IVF-ET unit.
- Gametes and embryos were processed using a culture medium containing penicillin and streptomycin.
- Microbial presence was monitored at various stages, and fertilization, cleavage, and pregnancy rates were assessed.
Main Results:
- Microorganisms were isolated from 50% of IVF-ET cycles.
- Potential sources included semen, oocyte collection needle washes, and follicular fluid.
- No significant impact on fertilization, cleavage, or pregnancy rates was observed despite microbial presence.
Conclusions:
- Seminal fluid and oocytes are identified as primary sources of microbial contamination in IVF-ET.
- Penicillin and streptomycin in culture media effectively mitigate microbial contamination.
- Commensal microbial contamination did not adversely affect key IVF-ET outcome measures.