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Related Experiment Videos

Gentamicin distribution from a collagen carrier

S Mehta1, J S Humphrey, D I Schenkman

  • 1Division of Orthopaedic Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|September 1, 1996
PubMed
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This study shows gentamicin-loaded bovine collagen effectively delivers antibiotics locally in rabbits. It maintains high local gentamicin levels while minimizing systemic exposure for 28 days.

Area of Science:

  • Biomaterials Science
  • Pharmacology
  • Infectious Diseases

Background:

  • Local antibiotic delivery via degradable carriers offers high concentrations and avoids systemic toxicity.
  • Degradable carriers may eliminate the need for intravenous access, renal monitoring, and surgical removal.

Purpose of the Study:

  • To evaluate the in vivo elution of gentamicin from processed bovine collagen (type I).
  • To assess local tissue and serum gentamicin concentrations over 28 days in rabbits.

Main Methods:

  • Processed bovine collagen (type I) impregnated with gentamicin (3 mg/kg) was implanted into the vastus lateralis of 66 rabbits.
  • Local tissue biopsies and serum samples were collected from 15 minutes to 28 days post-implantation.
  • Gentamicin concentrations were measured in local tissue and serum.

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Main Results:

  • Local tissue gentamicin levels averaged over 3,800 µg/ml in the first 4 hours, remaining above 2.70 µg/ml through day 28.
  • Serum gentamicin peaked at 4.04 µg/ml at 5 hours and remained below 0.41 µg/ml after 24 hours.
  • The carrier demonstrated effective gentamicin elution, maintaining therapeutic local concentrations and sub-toxic systemic levels.

Conclusions:

  • Gentamicin-impregnated bovine collagen is an effective degradable carrier for local antibiotic delivery.
  • This method provides sustained local gentamicin concentrations above the minimum inhibitory concentration.
  • The carrier ensures serum concentrations remain below toxic levels for 28 days post-implantation.