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Simultaneous obtention of an intense G-banding and chromosome painting
1UMR 147, Cytogénétique Moléculaire et Oncologie CNRS-Institut Curie, Paris, France. frichard@curie.fr
Cytogenetics and Cell Genetics
|January 1, 1996
Abstract:
A method for simultaneously obtaining brilliant G-banding and chromosome painting is described. It is based on a modification of methods enabling the detection of incorporated BrdU by anti-BrdU antibodies conjugated to a fluorochrome. It induces a banding of both painted and non-painted chromosomes. The method was adapted for in situ hybridizations on human and non human (ZOO-FISH) chromosomes.