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Related Experiment Videos

Ruminant cluster CD14

P Berthon1, J Hopkins

  • 1INRA, Laboratoire de Pathologie Infectieuse et Immunologie, Nouzilly, France. berthon@tours.inra.fr

Veterinary Immunology and Immunopathology
|August 1, 1996
PubMed
Summary
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Three monoclonal antibodies (mAbs) are specific for ruminant CD14. Another mAb recognizes a conserved bovine CD14 epitope, aiding in immune cell research.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD14 is a monocyte cell surface molecule.
  • Previous research has identified sheep and bovine CD14 homologues.

Purpose of the Study:

  • To characterize monoclonal antibodies (mAbs) specific for ruminant CD14.
  • To identify mAbs that recognize conserved CD14 epitopes between species.

Main Methods:

  • Flow cytometry analysis of ovine and bovine cells.
  • Immunoprecipitation, immunohistochemistry, and competitive binding studies.
  • Analysis of human CD14-transfected COS-7 cells and bovine peripheral blood mononuclear cells.

Main Results:

  • A cluster of three mAbs (VPM65, VPM66, VPM67) demonstrated specificity for ruminant CD14.

Related Experiment Videos

  • A fourth mAb (CC-G33) recognized a bovine CD14 epitope conserved in human CD14.

    • Conclusions:

    • The identified mAbs are valuable tools for studying ruminant CD14.
    • The conserved epitope recognized by CC-G33 offers potential for cross-species immunological applications.