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Updated: Sep 21, 2026
Catheterization of Intestinal Loops in Ruminants
Published on: June 10, 2009
Ruminant cluster CD62L
1Institute for Animal Health, Compton Laboratory, Newbury, UK.
Within the Third Workshop, four monoclonal antibodies (mAbs) were considered to belong to this cluster: IAH-CC32 (3W-360), BAQ92 (3W-485), Buf44 (3W-452) and IVA94 (3W-563). IAH-CC32 was the workshop control and has previously been identified as a CD62L-specific mAb (Howard, C.J., Sopp, P. and Parsons, K.R., 1992. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunol., 77:228-234) by showing that human L-selectin mAb LAM1.4 (Spertini, O., Kansas, G.S., Reimann, K.A., Mackay, C.R. and Tedder, T.F., 1991. Functional and evolutional characteristics of distinct epitopes on the leukocyte adhesion molecule-1 (TQ-1, Leu-8) that regulate leukocyte migration. J. Immunol., 147:942) cross-reacts with bovine cells and blocks binding of IAH-CC32. The mAb DREG-56 to human CD62L (Bosworth, 1993. Bovine L-selectin: a peripheral lymphocyte homing receptor. Vet., Immunol., Immunopathol, 37:201-215) also stains cattle cells. Buf44 blocked binding of IVA94 and IAH-CC32, indicating that all three bind to the same or closely related epitope on the L-selectin molecule (Naessens, 1996. Biochemical analysis of preliminary clusters in the non-lineage panel. Vet. Immunol. Immunopathol., 52: 347-356).
Within the Third Workshop, four monoclonal antibodies (mAbs) were considered to belong to this cluster: IAH-CC32 (3W-360), BAQ92 (3W-485), Buf44 (3W-452) and IVA94 (3W-563). IAH-CC32 was the workshop control and has previously been identified as a CD62L-specific mAb (Howard, C.J., Sopp, P. and Parsons, K.R., 1992. L-selectin expression differentiates T cells isolated from different lymphoid tissues in cattle but does not correlate with memory. Immunol., 77:228-234) by showing that human L-selectin mAb LAM1.4 (Spertini, O., Kansas, G.S., Reimann, K.A., Mackay, C.R. and Tedder, T.F., 1991. Functional and evolutional characteristics of distinct epitopes on the leukocyte adhesion molecule-1 (TQ-1, Leu-8) that regulate leukocyte migration. J. Immunol., 147:942) cross-reacts with bovine cells and blocks binding of IAH-CC32. The mAb DREG-56 to human CD62L (Bosworth, 1993. Bovine L-selectin: a peripheral lymphocyte homing receptor. Vet., Immunol., Immunopathol, 37:201-215) also stains cattle cells. Buf44 blocked binding of IVA94 and IAH-CC32, indicating that all three bind to the same or closely related epitope on the L-selectin molecule (Naessens, 1996. Biochemical analysis of preliminary clusters in the non-lineage panel. Vet. Immunol. Immunopathol., 52: 347-356).
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