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Identification of bovine CD14
P Sopp1, L S Kwong, C J Howard
1Institute for Animal Health, Compton Laboratory, Newbury, UK.
Veterinary Immunology and Immunopathology
|August 1, 1996
Summary
Monoclonal antibodies (mAbs) were tested against human CD14. Only one mAb, CC-G33, recognized the human molecule, while three others targeted bovine CD14.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Monoclonal antibodies (mAbs) are crucial tools in immunology.
- Characterizing antibody specificity is essential for their application.
Purpose of the Study:
- To determine the specificity of six mAbs against human CD14.
- To identify which mAbs recognize the human CD14 molecule.
Main Methods:
- COS-7 cells were transfected with cDNA encoding human CD14.
- Monoclonal antibodies were tested for their ability to stain transfected cells.
- Flow cytometry was used to compare antibody binding.
Main Results:
- Only mAb CC-G33 recognized the human CD14 molecule.
- Three of the six tested mAbs were found to be directed against bovine CD14.
Conclusions:
- mAb CC-G33 is specific for human CD14.
- This study differentiated human CD14-specific mAbs from those targeting bovine CD14.