Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hamster antithrombin III: purification, characterization and acute phase response

P Mak1, J J Enghild, A Dubin

  • 1Institute of Molecular Biology, Jagiellonian University, Kraków, Poland.

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
|September 1, 1996
PubMed
Summary

Hamster antithrombin III (ATIII) was purified and characterized, revealing high similarity to other mammals. This protein effectively inhibits thrombin, with minimal changes during acute phase responses.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative genomic study of arachnid immune systems indicates loss of beta-1,3-glucanase-related proteins and the immune deficiency pathway.

Journal of evolutionary biology·2015
Same author

Prognostic value of brachioradialis muscle oxygen saturation index and vascular occlusion test in septic shock patients.

Medicina intensiva·2015
Same author

Oxytocinergic regulation of endogenous as well as drug-induced mood.

Pharmacology, biochemistry, and behavior·2013
Same author

Physicochemical characterization of fish protein adlayers with bacteria repelling properties.

Colloids and surfaces. B, Biointerfaces·2012
Same author

SAXS models of TGFBIp reveal a trimeric structure and show that the overall shape is not affected by the Arg124His mutation.

Journal of molecular biology·2011
Same author

The diversity of FtsY-lipid interactions.

Biopolymers·2010

Area of Science:

  • Biochemistry
  • Proteomics
  • Mammalian Physiology

Background:

  • Antithrombin III (ATIII) is a crucial anticoagulant protein.
  • Understanding species-specific variations in ATIII is important for comparative physiology and drug development.

Purpose of the Study:

  • To purify and characterize antithrombin III from hamster plasma.
  • To compare hamster ATIII properties with those of other mammals.

Main Methods:

  • Affinity chromatography (heparin-agrose), ion-exchange chromatography (Mono Q), and size-exclusion chromatography (TSK G3000SWG) were used for purification.
  • Molecular mass, absorption coefficient, isoelectric point (pI), N-terminal amino acid sequencing, immunological cross-reactivity, and kinetic inhibition assays were performed.

Related Experiment Videos

Main Results:

  • Hamster ATIII was purified with 50% yield, showing a molecular mass of 62.5 kDa and pI range of 4.95-5.25.
  • N-terminal sequencing revealed 84% identity to mouse and 76% to human ATIII.
  • Hamster ATIII exhibited low cross-reactivity with anti-human ATIII antibodies and high efficiency in inhibiting bovine and human thrombin, with minimal changes during acute phase response.

Conclusions:

  • Hamster antithrombin III shares significant structural and functional similarities with antithrombins from other mammals.
  • These findings contribute to the comparative understanding of anticoagulation mechanisms across species.