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Updated: Aug 15, 2026
Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
Effect of volume variations on osteogenic capabilities of autogenous cancellous bone graft in dogs
W J DeVries1, C L Runyon, S A Martinez
1Department of Companion Animals, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.
Objective:
To evaluate the osteogenic effect of differing volumes of autogenous cancellous bone graft (ACBG) placed into partial cortical defects of the ulna.
Animals:
15 healthy, mature Beagles.
Procedure:
Weekly radiographic views of defects were obtained over 8 weeks and were analyzed for bone density by use of radiographic optical densitometry. Histologic sections were obtained 8 weeks after surgery and were planimetrically evaluated for area of total, lamellar, and woven bone.
Results:
Defects receiving 0.3 and 0.75 g of ACBG had rapid initial bone production, and dogs receiving 0.3 g of ACBG had faster bone ingrowth than did those receiving 0.1 g of ACBG or controls. Defects receiving 0.75 g of ACBG had a rate of bony ingrowth equal to 0.3 g of ACBG. There was no difference in the histomorphometric area fractions of total, lamellar, or woven bone between defects treated with 0.1 or 0.75 g of ACBG, and between grafted or control defects, 8 weeks after surgery.
Conclusions:
Overfilling a cortical defect with ACBG does not enhance early osteogenesis within the defect, and underfilling will delay the onset of osteogenesis within the defect.
Clinical Relevance:
A volume of ACBG sufficient only to fill a cortical defect is required to obtain a clinical osteogenic effect from the graft.
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