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Protein kinase C inactivation by Fenton's-reaction at discrete CU++ binding sites
N Traverso1, R Ricciarelli, C Domenicotti
1Institute of General Pathology, University of Genoa, Italy.
Biochemistry and Molecular Biology International
|October 1, 1996
Abstract:
The consequence of direct exposure to HO. radical (chemically generated by Fenton's reaction) of partially purified rat liver PKC has been evaluated in this work. PKC inhibited Fenton-dependent HO. generation, probably due to the binding of copper ions to the enzyme. PKC activity was inhibited by H2O2. Copper ions were able to increase the H2O2-mediated damage to the enzyme, but only beyond a concentration threshold. The possible interactions between PKC and Fenton's reagents, in particular copper ions, is discussed.