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Hypothalamic cholinergic activity associated with 2-deoxyglucose-induced hyperglycemia
A Takahashi1, H Ishimaru, Y Ikarashi
1Department of Neuropsychopharmacology, (Tsumura), Gunma University School of Medicine, Japan.
Brain Research
|September 23, 1996
Summary
Administration of 2-deoxyglucose (2-DG) increases hypothalamic cholinergic activity, leading to hyperglycemia. This suggests the hypothalamic cholinergic system plays a role in regulating blood glucose levels.
Area of Science:
- Neuroscience
- Endocrinology
- Metabolism
Background:
- The hypothalamus regulates peripheral glucose metabolism.
- The role of the hypothalamic cholinergic system in this regulation is not fully understood.
Purpose of the Study:
- To investigate the involvement of the hypothalamic cholinergic system in 2-deoxyglucose (2-DG)-induced hyperglycemia.
- To clarify the function of hypothalamic acetylcholine (ACh) in glucose homeostasis.
Main Methods:
- Rats were administered 2-deoxyglucose (2-DG) intravenously.
- Brain microdialysis was used to measure extracellular acetylcholine (ACh) and choline levels in the hypothalamus.
- Tissue levels of ACh and choline in the ventromedial hypothalamic nucleus (VMH) and lateral hypothalamus (LH) were measured.
- Experiments were conducted in anesthetized and 6-hydroxydopamine (6-OHDA) pretreated rats.
Main Results:
- 2-DG administration caused significant hyperglycemia and increased extracellular ACh and choline levels in the hypothalamus.
- Hypothalamic tissue showed decreased ACh and increased choline content after 2-DG administration.
- Anesthesia and 6-OHDA pretreatment (reducing norepinephrine) attenuated the hyperglycemic response and hypothalamic cholinergic changes.
- These findings indicate increased hypothalamic cholinergic activity following 2-DG administration.
Conclusions:
- The hypothalamic cholinergic system is involved in 2-DG-induced hyperglycemia.
- Acetylcholine in the hypothalamus plays a role in the regulation of peripheral glucose metabolism.