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Specificity of IS6110-based amplification assays for Mycobacterium tuberculosis complex
T J Hellyer1, L E DesJardin, M K Assaf
1Department of Pathology, University of Arkansas for Medical Sciences, John L. McClellan Memorial Veterans' Hospital, Little Rock.
Journal of Clinical Microbiology
|November 1, 1996
Abstract:
The specificity of IS6110 for the Mycobacterium tuberculosis complex has recently been questioned. We observed no cross-reaction with 27 nontuberculous mycobacteria using strand displacement- and PCR-based amplification of the nucleotide 970 to 1026 and 762 to 865 regions of IS6110. These data support use of selected regions of IS6110 as M. tuberculosis complex-specific targets.