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Parietal cell MAP kinases: multiple activation pathways

K Nakamura¹, C J Zhou, J Parente

¹Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912-3175, USA.

The American Journal of Physiology

|October 1, 1996

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Epidermal growth factor (EGF) biphasically activates extracellular signal-regulated kinases (ERKs) 1 and 2 in gastric parietal cells, influencing acid secretion. This pathway is distinct from phosphatidylinositol 3-kinase signaling.

Area of Science:

Cellular signaling
Gastroenterology
Molecular biology

Background:

Epidermal growth factor (EGF) exhibits a complex, biphasic effect on gastric parietal cell HCl secretion, involving initial inhibition followed by sustained enhancement.
Mitogen-activated protein kinase (MAPK) pathways are implicated in cellular responses to growth factors, but their role in highly differentiated parietal cells is not fully understood.

Purpose of the Study:

To investigate the presence and activation mechanisms of MAPK isoforms, specifically extracellular signal-regulated kinases (ERKs) 1 and 2, in gastric parietal cells.
To determine the role of ERK activation in mediating the acute and sustained effects of EGF on parietal cell acid secretion.

Main Methods:

Primary culture of rabbit gastric parietal cells.
Analysis of ERK 1 and 2 activation in response to EGF, phorbol ester (TPA), carbachol, ionomycin, and forskolin.

Related Experiment Videos

Investigation of signaling pathways including protein kinase C, phosphatidylinositol 3-kinase, intracellular calcium, and cyclic AMP.

Main Results:

EGF biphasically activated ERK 1 and 2 in parietal cells, with activation kinetics mirroring EGF's effect on acid secretion.
TPA and carbachol also activated ERKs, but through distinct patterns and potentially overlapping pathways.
ERK activation was not modulated by intracellular calcium or cAMP levels, and the phosphatidylinositol 3-kinase pathway did not appear to regulate ERK signaling in these cells.

Conclusions:

EGF activates ERKs in gastric parietal cells, contributing to the regulation of acid secretion.
Parietal cells utilize distinct signaling pathways for EGF, TPA, and carbachol.
The ERK signaling cascade in parietal cells is not directly influenced by phosphatidylinositol 3-kinase, calcium, or cAMP.