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Time course of luciferyl adenylate synthesis in the firefly luciferase reaction
A Dukhovich1, A Sillero, M A Sillero
1Departamento de Bioquímica, Instituto de Investigaciones Biomedicas del CSIC, Universidad Autónoma de Madrid, Spain.
FEBS Letters
|October 21, 1996
Abstract:
The time course of luciferyl adenylate formation in the reaction catalyzed by firefly luciferase (EC 1.13.12.7) has been followed. The properties of luciferyl adenylate, enzymatically or chemically synthesized, as substrate of luciferase, have been compared. The potential use of luciferyl adenylate for luciferase detection is here proposed.