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Distinct spatial and temporal functions for PS integrins during Drosophila wing morphogenesis
M C Brabant1, D Fristrom, T A Bunch
1Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721, USA.
Development (Cambridge, England)
|October 1, 1996
Summary
Integrins in Drosophila wings have early and late functions in morphogenesis. Early functions involve signaling, while late functions relate to cell-matrix adhesion, maintaining wing layer association.
Area of Science:
- Developmental Biology
- Cell Biology
- Genetics
Background:
- Integrins are crucial cell-surface receptors involved in cell-matrix adhesion.
- In Drosophila wing development, integrins PS1 and PS2 show distinct dorsal and ventral expression patterns.
- Previous studies suggested integrins maintain wing surface association.
Purpose of the Study:
- To investigate the specific roles of integrins in Drosophila wing morphogenesis.
- To differentiate between early and late functions of integrins during pupariation.
- To understand the signaling roles of integrins in wing development.
Main Methods:
- Clonal analysis of integrin-deficient cells in Drosophila wings.
- Observation of wing layer apposition and re-apposition during metamorphosis.
- Examination of integrin organization in basal plaques.
- Overexpression studies of alphaPS subunits.
Main Results:
- Wing layers appose early in metamorphosis even without integrins.
- Integrin mutant clones fail to re-appose later in pupation.
- Dorsoventral segregation of PS1 and PS2 appears necessary for some integrin functions.
- Overexpression of alphaPS subunits causes wing blistering at pupariation onset.
Conclusions:
- Integrin functions in wing morphogenesis can be divided into early (signaling) and late (adhesion) roles.
- Early integrin functions likely involve dorsoventral segregation and signaling pathways.
- Late integrin functions align with their known roles in cell-matrix adhesion.