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Streptomycin: separation of polysomal and non-polysomal messenger ribonucleoproteins
Biochimica Et Biophysica Acta
|September 6, 1977
Abstract:
In the presence of 0.02 M streptomycin, all of the polysomes precipitate from male cricket (Acheta domesticus) accessory gland and chick embryonic tissue post-mitochondrial fractions. All non-polysomal messenger-like ribonucleoprotein preparations tested remain in solution.
Insights
Streptomycin causes polysomes to precipitate from cell fractions. Non-polysomal messenger-like ribonucleoprotein complexes remain soluble, aiding in their separation.
Area of Science:
- Molecular Biology
- Biochemistry
Background:
- Polysomes are key to protein synthesis.
- Understanding polysome behavior is crucial for studying gene expression.
Purpose of the Study:
- To investigate the effect of streptomycin on polysome precipitation.
- To differentiate polysomal from non-polysomal messenger-like ribonucleoprotein complexes.
Main Methods:
- Utilizing post-mitochondrial fractions from male cricket accessory gland and chick embryonic tissue.
- Treating fractions with 0.02 M streptomycin.
- Observing precipitation and solubility of different ribonucleoprotein complexes.
Main Results:
- Streptomycin induced complete precipitation of all polysomes.
- Non-polysomal messenger-like ribonucleoprotein preparations remained soluble in the presence of streptomycin.
Conclusions:
- Streptomycin can be used to selectively precipitate polysomes.
- This method allows for the separation and study of polysomal and non-polysomal messenger-like ribonucleoprotein complexes.