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Annexins and protein kinases C
T Dubois1, J P Oudinet, J P Mira
1INSERM U 332, Institut Cochin de Génétique Moléculaire (ICGM), Paris, France.
Biochimica Et Biophysica Acta
|October 11, 1996
Summary
Annexins and protein kinases C are key signaling proteins. Annexin V uniquely modulates protein kinase C activity, potentially regulating other annexins.
Area of Science:
- Molecular Biology
- Cell Signaling
- Biochemistry
Background:
- Annexins and Protein Kinases C (PKCs) are ubiquitous cytoplasmic proteins crucial for signal transduction.
- Annexins bind calcium and phospholipids, while PKCs have diverse activation requirements (calcium, diacylglycerol, phospholipids).
- PKCs regulate numerous cellular processes through phosphorylation.
Purpose of the Study:
- To investigate the interaction between annexins and Protein Kinases C.
- To elucidate the specific role of annexin V in PKC signaling pathways.
- To explore the potential substrate relationship between annexin V and other annexins.
Main Methods:
- In vitro and in vivo assays to assess annexin phosphorylation by PKCs.
- Analysis of putative binding and pseudo-substrate sites for PKCs on annexins.
- Functional studies to determine annexin V's effect on PKC activity.
Main Results:
- Most annexins serve as substrates for PKCs in vitro and in vivo.
- Annexin V is an exception, lacking a direct substrate role but possessing a potential pseudo-substrate site for PKCs.
- Annexins possess putative binding sites for PKCs.
Conclusions:
- Annexin V may act as a modulator, regulating the activity of conventional PKCs (cPKCs) towards other annexin substrates.
- This interaction suggests a novel regulatory feedback mechanism within annexin-PKC signaling networks.
- Understanding this cross-talk is vital for comprehending cellular signal transduction.