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Use of the direct RNA amplification technique NASBA to detect factor V Leiden, a point mutation associated with APC
P H Reitsma1, P A van der Velden, E Vogels
1Department of Hematology, University Hospital, Leiden, The Netherlands.
Abstract:
APC resistance is a common and strong hereditary risk factor for venous thrombosis. This plasma abnormality appears to be almost always caused by the same defect in the coagulation factor V gene (a G --> A transition at nucleotide 1691 leading to replacement of 506 Arg by Gln; factor V Leiden). Therefore, it is possible to consider a simple and specific genetic test as an alternative to a plasma APC resistance test that is compromised by treatment and other factors. We have investigated whether a new amplification procedure, NASBA, together with the detection procedure ELGA would provide a simple protocol for the nucleotide specific detection of the factor V mutation.