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Related Experiment Videos

Keratokyphosis in rabbits: a functional and morphologic study

H Kruse1, H Heimann, A Schüler

  • 1Department of Ophthalmology, Steglitz Medical Center, Free University of Berlin, Germany.

Cornea
|November 1, 1996
PubMed
Summary

Keratokyphosis, a novel keratomileusis technique, induced significant refractive changes in rabbits. Corneal thickness and nerve density were altered, with potential implications for endothelial cell demand.

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Area of Science:

  • Ophthalmology
  • Corneal Surgery
  • Refractive Surgery

Background:

  • Keratokyphosis is a
  • nonfreeze keratomileusis
  • procedure.
  • Previous research on its effects is limited.

Purpose of the Study:

  • To evaluate the refractive and pachymetric outcomes of keratokyphosis in a rabbit model.
  • To assess corneal wound healing and nerve regeneration post-procedure.
  • To investigate the relationship between corneal thickness, endothelial cell morphology, and refractive outcomes.

Main Methods:

  • Keratokyphosis was performed on rabbit eyes using varying microkeratome applanation surfaces (convex and concave).
  • Refractive error (diopters) and corneal thickness (microns) were measured.

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  • Corneal wound healing, interface scar width, and subbasal nerve fiber density were assessed.
  • Endothelial cell density and size were analyzed using specular microscopy and vital preparation techniques.

    • Main Results:

    • Significant myopic refractive shifts were observed with convex applanation surfaces (-6.5 to -13.0 D).
    • Corneal pachymetry increased with convex surfaces (+39 to +71 microns) and decreased with concave surfaces (-17 to -21 microns).
    • Wound healing was uncomplicated with minimal interface scarring (0-5 microns).
    • Endothelial cell size increased significantly (25.1%, p < 0.01) compared to vital preparation, particularly with higher refractive changes.
    • A negative correlation was found between endothelial cell count and corneal thickness (r = -0.63).

    Conclusions:

    • Keratokyphosis effectively alters refractive error in rabbits.
    • The procedure appears safe with uncomplicated wound healing.
    • Thickened corneas may increase the metabolic demand on the corneal endothelium.