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Purification and characterization of enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4
H M Joosten1, M Nunez, B Devreese
1Departamento de Tecnología de Alimentos, CIT-INIA, Madrid, Spain. han.joosten@chlsnr.nestrd.ch
Applied and Environmental Microbiology
|November 1, 1996
Abstract:
A simple two-step procedure was developed to obtain pure enterocin 4, a bacteriocin produced by Enterococcus faecalis INIA 4. Chemical and genetic characterization revealed that the primary structure of enterocin 4 is identical to that of peptide antibiotic AS-48 from Enterococcus faecalis S-48. In contrast to the reported inhibitory spectrum of AS-48, enterocin 4 displayed no activity against gram-negative bacteria.