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The fate of Mn2+ ions inside Saccharomyces cerevisiae cells seen by electron paramagnetic resonance

I C Farcasanu1, N Ohta, T Miyakawa

  • 1Department of Fermentation Technology, Hiroshima University, Japan.

Bioscience, Biotechnology, and Biochemistry
|March 1, 1996
PubMed

Insights

Manganese (Mn2+) rapidly precipitates in Saccharomyces cerevisiae cells upon import. Only soluble manganese ions are exported, while bound manganese must first become soluble to exit the cell.

Area of Science:

  • Cellular biology
  • Biochemistry
  • Biophysics

Background:

  • Manganese (Mn2+) is an essential trace element for Saccharomyces cerevisiae.
  • Understanding manganese transport is crucial for cellular homeostasis and toxicity prevention.

Purpose of the Study:

  • To investigate the intracellular fate of manganese ions during import and export in yeast cells.
  • To elucidate the mechanisms governing manganese homeostasis in Saccharomyces cerevisiae.

Main Methods:

  • Utilized electron paramagnetic resonance (EPR) spectroscopy to monitor Mn2+.
  • Analyzed EPR spectra under conditions of manganese import and efflux.

Main Results:

  • High intracellular concentrations of Mn2+ led to immediate precipitation within yeast cells.
  • EPR data indicated that only soluble Mn2+ ions were exported.
  • Bound manganese required conversion to a soluble form before cellular export.

Conclusions:

  • Manganese homeostasis in Saccharomyces cerevisiae involves intracellular precipitation to manage high influx.
  • Cellular export of manganese is a regulated process dependent on the ion's soluble state.

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