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Calibration of the Ektachem Amylase method using human reference materials and the Phadebas Blue Starch method as an
P K Mogensen1, J Møller, L Nyborg
1Department of Clinical Chemistry, Herning Centralsygehus, Denmark.
Scandinavian Journal of Clinical and Laboratory Investigation
|October 1, 1996
Summary
This study calibrated the Ektachem Amylase method to match Phadebas Blue Starch results, reducing lab variation by 40%. A consistent calibration value for the Nordic Amylase Calibrator was recommended for Ektachem analyzers.
Area of Science:
- Clinical Chemistry
- Enzyme Assays
- Laboratory Medicine
Background:
- Standardization of amylase assays is crucial for accurate diagnosis.
- Existing methods showed interlaboratory variability.
- The Ektachem Amylase method required calibration against a reference method.
Purpose of the Study:
- To establish a consensus calibration for the Ektachem Amylase method.
- To ensure reproducibility with the Phadebas Blue Starch method.
- To reduce interlaboratory variation in amylase measurements.
Main Methods:
- Multilaboratory study involving calibration.
- Development of a calibration graph (slope=3.39, intercept=25).
- Calculation of reaction-rate ratios for salivary and pancreatic amylase.
Main Results:
- Consensus calibration reduced interlaboratory variation by up to 40%.
- Ektachem Amylase method showed a reaction-rate ratio of 0.89 relative to Phadebas.
- Recommended using the stated value (460 U l-1) for the Nordic Amylase Calibrator.
Conclusions:
- Consensus calibration significantly improves amylase assay consistency.
- The Ektachem Amylase method, when calibrated, provides reliable results.
- Standardization efforts are vital for accurate clinical enzyme measurements.