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Related Experiment Videos

P-selectin induction of tissue factor biosynthesis and expression

B Furie1, B C Furie

  • 1Center for Hemostasis and Thrombosis Research, Division of Hematology-Oncology, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

Haemostasis
|January 1, 1996
PubMed
Summary

P-selectin on activated platelets and endothelial cells promotes monocyte and neutrophil adhesion. This study shows P-selectin directly upregulates tissue factor expression on monocytes, a key factor in blood clotting.

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A journey with platelet P-selectin: the molecular basis of granule secretion, signalling and cell adhesion.

Thrombosis and haemostasis·2001

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • P-selectin is expressed on activated platelets and endothelial cells, mediating cell adhesion.
  • Activated platelets are known to induce tissue factor (TF) on monocytes.
  • The specific role of P-selectin in TF induction on monocytes was not fully understood.

Purpose of the Study:

  • To investigate the effect of P-selectin on the induction of tissue factor activity in monocytes.
  • To determine if P-selectin directly influences TF expression on the monocyte surface.

Main Methods:

  • Utilized Chinese hamster ovary (CHO) cells engineered to express P-selectin.
  • Assessed TF activity in purified monocytes incubated with P-selectin-expressing CHO cells.
  • Employed anti-P-selectin antibodies to block P-selectin function.

Related Experiment Videos

  • Measured TF mRNA and TF antigen expression on monocytes.

    • Main Results:

    • CHO cells expressing P-selectin stimulated TF activity in purified monocytes.
    • Unmodified CHO cells and CHO cells expressing E-selectin did not induce TF activity.
    • Anti-P-selectin antibodies significantly inhibited the P-selectin-mediated TF induction.
    • P-selectin stimulation led to increased TF mRNA and TF antigen on monocytes.

    Conclusions:

    • P-selectin plays a direct role in upregulating tissue factor expression on monocytes.
    • This interaction highlights a novel mechanism linking P-selectin to procoagulant activity in monocytes.