Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

c-Fos expression during wakefulness and sleep

M Pompeiano1, C Cirelli, P Arrighi

  • 1Istituto di Chimica Biologica, Universita di Pisa, Italy.

Neurophysiologie Clinique = Clinical Neurophysiology
|January 1, 1995
PubMed
Summary

We found that c-fos gene expression increases with wakefulness in brain regions regulating arousal. This gene is causally linked to sleep regulation, particularly in the medial preoptic area.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-density EEG signature of NREM sleep parasomnia episodes.

Scientific reports·2026
Same author

Network alterations in temporal lobe epilepsy during non-rapid eye movement sleep and wakefulness.

Clinical neurophysiology : official journal of the International Federation of Clinical Neurophysiology·2024
Same author

The cerebellum monitors errors and entrains executive networks.

Brain research·2023
Same author

Correction to: Adverse effects of sodium‑glucose cotransporter‑2 inhibitors in patients with heart failure: a systematic review and meta‑analysis.

Heart failure reviews·2023
Same author

Adverse effects of sodium-glucose cotransporter-2 inhibitors in patients with heart failure: a systematic review and meta-analysis.

Heart failure reviews·2023
Same author

Cerebral hemorrhage related to vein thrombosis in Covid-19 patients in different Italian hospitals: View point for clinical and imaging implications.

Journal of the neurological sciences·2020

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Sleep Science

Background:

  • c-fos gene expression is a marker for neuronal activity.
  • Wakefulness and sleep are fundamental biological processes regulated by complex neural circuits.
  • Understanding the molecular mechanisms underlying the sleep-wake cycle is crucial for addressing sleep disorders.

Purpose of the Study:

  • To investigate the role of c-fos gene expression in the regulation of sleep and wakefulness.
  • To identify specific brain regions where c-fos expression is modulated by arousal states.
  • To establish a causal link between c-fos expression in key brain areas and sleep regulation.

Main Methods:

  • Measuring c-fos expression in various brain regions of rodents under spontaneous and forced wakefulness conditions.

Related Experiment Videos

  • Utilizing c-fos antisense injections in the medial preoptic area to assess the causal role of c-fos in sleep regulation.
  • Analyzing c-fos expression patterns in the cerebral cortex and hippocampus.

    • Main Results:

    • c-fos expression is significantly induced by both spontaneous and forced wakefulness across multiple brain regions.
    • Elevated c-fos expression was observed in arousal-regulating areas, including the locus coeruleus and medial preoptic area.
    • c-fos antisense injection in the medial preoptic area demonstrated its causal involvement in sleep regulation.
    • Increased c-fos expression in the cerebral cortex and hippocampus may reflect the functional consequences of prolonged wakefulness.

    Conclusions:

    • c-fos expression is a key molecular indicator of neuronal activity during wakefulness and is implicated in sleep regulation.
    • The medial preoptic area plays a critical role in sleep regulation, mediated in part by c-fos expression.
    • Further research is needed to elucidate the precise mechanisms of c-fos and its target genes in the sleep-wake cycle.