[Na+/K+/2Cl- cotransport protein, Na+/Cl- cotransport protein]
T Inoue1, H Nonoguchi, K Tomita
1Third Department of Internal Medicine, Kumamoto University School of Medicine, Japan.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|March 1, 1996
Summary
The bumetanide-sensitive Na+/K+/2Cl- cotransporter and thiazide-sensitive Na+/Cl- cotransporter are key for cell volume and salt transport. Their similar structure, revealed by cDNA cloning, suggests they belong to a new transporter family.
Area of Science:
- Biochemistry
- Cell Biology
- Physiology
Context:
- The bumetanide-sensitive Na+/K+/2Cl- cotransporter (NKCC) and thiazide-sensitive Na+/Cl- cotransporter (NCC) are crucial ion transporters found in many tissues.
- These transporters play vital roles in physiological processes like cell volume regulation and salt homeostasis.
Purpose:
- To investigate the structural and familial relationship between NKCC and NCC.
- To understand the functional significance of their conserved topology and regulatory mechanisms.
Summary:
- Recent cDNA cloning reveals that NKCC and NCC share a conserved topology with 12 transmembrane helices.
- This structural similarity suggests they are members of a novel cotransporter family.
- Both transporters are regulated by phosphorylation and dephosphorylation, potentially influenced by intracellular chloride levels.
Impact:
- Elucidates the molecular basis of ion transport and cell volume control.
- Provides insights into the evolution and classification of membrane transport proteins.
- Advances understanding of physiological salt balance and potential therapeutic targets.
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