The substrate attached material synthesized by clonal cell lines of nerve, glia, and muscle

A large number of clonal cell lines were examined for the deposition of proteins (substrate attached material, SAM) on the substratum of culture dishes. Of the many cell types examined, only nerve, muscle, and glia synthesized SAM containing a predominant species of protein with a apparent molecular weight of 55,000. This 55,000 molecular weight protein is distinguishable from actin, tubulin, and collagen.