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Bone Conditioned Medium: Preparation and Bioassay
Published on: July 8, 2015
Aging effect on inductive capacity of human demineralized bone matrix
C Nyssen-Behets1, O Delaere, P Y Duchesne
1Institut d'Anatomie, Université de Mons-Hainaut, Mons, Belgium.
Archives of Orthopaedic and Trauma Surgery
|January 1, 1996
Summary
Human demineralized bone powder (DBP) shows limited osteoinductivity compared to other species. Age impacts alkaline phosphatase activity, indicating potential age-related bone matrix alterations affecting osteogenesis.
Area of Science:
- Biomaterials Science
- Orthopedic Research
- Tissue Engineering
Background:
- Demineralized bone powder (DBP) is utilized in bone regeneration.
- Human DBP's osteoinductive potential requires further investigation, especially concerning donor age.
- Age-related changes in bone matrix may influence osteogenic capacity.
Purpose of the Study:
- To evaluate the osteoinductive capacity of human demineralized bone powder (DBP).
- To assess the influence of donor age on the osteoinductive properties of human DBP.
- To investigate the relationship between histomorphometry and biochemical markers of osteogenesis.
Main Methods:
- Human cortical bone was processed into demineralized bone powder (DBP).
- DBP was implanted into athymic Nu/Nu mice for 28 days.
- Osteoinductivity was assessed via histomorphometry and alkaline phosphatase activity.
Main Results:
- Human DBP demonstrated limited new bone and cartilage formation.
- Histomorphometric data showed no significant difference between young and aged donors.
- Alkaline phosphatase activity was significantly lower in implants from aged donors.
Conclusions:
- Human DBP exhibits lower osteoinductivity compared to DBP from other species.
- Donor age may affect osteoblastic activity, indicated by reduced alkaline phosphatase in aged bone DBP.
- Age-related modifications in bone matrix proteins, like bone morphogenetic protein, could underlie decreased osteoinductive capacity.
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