Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between endothelial cells and effector cells in allergic inflammation

A B Tonnel1, P Gosset, S Molet

  • 1Unité INSERM n degree 416, Institut Pasteur Lille, France.

Annals of the New York Academy of Sciences
|October 31, 1996
PubMed
Summary

Endothelial cells (EC) play a key role in lung inflammation by interacting with inflammatory cells. Histamine and eosinophils significantly influence EC responses, impacting adhesion molecule expression and cytokine production in allergic reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Updated guidelines (2015) for management and monitoring of adult and adolescent asthmatic patients (from 12 years and older) of the Société de Pneumologie de Langue Française (SPLF) (Full length text).

Revue des maladies respiratoires·2016
Same author

Updated guidelines (2015) for management and monitoring of adult and adolescent asthmatic patients (from 12 years and older) of the Société de pneumologie de langue française (SPLF) (summary).

Revue des maladies respiratoires·2016
Same author

From parasites to allergy: a second receptor for IgE.

Immunology today·2014
Same author

[Features of asthma in women: what is the relationship with hormonal status?].

Revue des maladies respiratoires·2014
Same author

[Lung is also involved in juvenile dermatomyositis].

La Revue de medecine interne·2014
Same author

Oxidative stress-mediated iNKT-cell activation is involved in COPD pathogenesis.

Mucosal immunology·2013

Area of Science:

  • Immunology
  • Cell Biology
  • Pulmonology

Background:

  • Local inflammatory responses in the lungs involve complex interactions between inflammatory and resident structural cells.
  • Endothelial cells (EC) are critical structural cells that regulate leukocyte traffic and amplify leukocyte activation via cytokine and chemokine production.

Purpose of the Study:

  • To investigate the role of endothelial cells (EC) in the inflammatory response within the lung.
  • To examine how inflammatory cells like macrophages, mast cells, and eosinophils influence EC behavior.

Main Methods:

  • Cultured human umbilical vein endothelial cells (HUVEC) were exposed to supernatants from alveolar macrophages of asthmatic patients.
  • HUVEC were treated with histamine, a mast cell mediator, and histamine receptor antagonists.

Related Experiment Videos

  • HUVEC were exposed to purified eosinophils from patients with hypereosinophilia.

    • Main Results:

    • Macrophage supernatants induced TNF alpha-dependent overexpression of ICAM-1 and E-selectin on HUVEC.
    • Histamine induced concentration-dependent IL-6 and IL-8 secretion by HUVEC, inhibited by H1/H2 receptor antagonists.
    • Eosinophils increased adhesion molecule expression and chemokine production by HUVEC, with specific mediators under investigation.

    Conclusions:

    • Endothelial cells are key players in the local inflammatory response in the lung, responding to mediators from macrophages, mast cells, and eosinophils.
    • Allergen-induced inflammatory cells modulate endothelial cell function, contributing to the late-phase asthmatic reaction.
    • Further research is needed to identify specific eosinophil products involved in endothelial cell activation.