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Insulin-like growth factor-II/mannose-6-phosphate receptor expression during early heart development

K M McCormick1, N M Dahms, J Lough

  • 1Department of Cellular Biology and Anatomy, Medical College of Wisconsin, Milwaukee 53226, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|October 1, 1996
PubMed
Summary

The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor is crucial for heart development. Its expression in chicken embryos increases significantly during early heart formation, particularly in the myocardium.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cardiovascular Research

Background:

  • The insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptor plays roles in growth and development.
  • Understanding its role in organogenesis, especially heart development, is essential.

Purpose of the Study:

  • To investigate the expression patterns of the IGF-II/M6P receptor during chicken heart morphogenesis.
  • To determine the specific role of this receptor in early cardiac development.

Main Methods:

  • Western blot analysis of embryonic proteins from stages 5-24.
  • Immunohistochemical localization of the IGF-II/M6P receptor in embryonic tissues.
  • In vitro model of cardiogenesis using explanted precardiac mesoderm and endoderm.

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Main Results:

  • IGF-II/M6P receptor protein was detected starting at stage 7, preceding myocardial tube formation.
  • Receptor levels increased until stage 14 and remained constant thereafter.
  • The developing myocardium was the primary site of receptor expression.
  • In vitro, receptor expression was observed only after differentiation into a contractile cardiac muscle.

Conclusions:

  • The IGF-II/M6P receptor is expressed in a temporally and spatially regulated manner during heart development.
  • These findings suggest a significant role for the IGF-II/M6P receptor in early heart development and differentiation.