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Related Concept Videos

Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Chromatographic methods for amylases

B M Brena1, C Pazos, L Franco-Fraguas

  • 1Cátedra de Bioquúmico, Facultad de Química e Instituto de Química, Facultad de Ciencias, Montevideo, Uruguay.

Journal of Chromatography. B, Biomedical Applications
|September 20, 1996
PubMed
Summary
This summary is machine-generated.

This review details new chromatographic techniques for separating amylases and their isozymes from complex samples. It also covers methods for determining amylase activity and provides data on alpha- and beta-amylases.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry

Background:

  • Amylases are crucial enzymes with diverse applications.
  • Separating amylases from complex biological matrices presents significant challenges.
  • Isozymes of amylase require specific separation techniques.

Purpose of the Study:

  • To review recent advancements in chromatographic methods for amylase separation.
  • To evaluate the application of new chromatographic techniques for amylase purification.
  • To provide an updated overview of amylase activity determination methods.

Main Methods:

  • Survey of recent literature on chromatographic separation techniques.
  • Detailed evaluation of newly developed chromatographic methods.
  • Compilation of data on amylase properties and characteristics.

Main Results:

  • Recent chromatographic methods offer improved separation of amylases and isozymes.
  • New techniques enhance the purification efficiency of amylases from complex extracts.
  • Updated information on amylase activity assays is presented.

Conclusions:

  • Advanced chromatographic methods are key for effective amylase and isozyme purification.
  • This review consolidates current knowledge on amylase separation and activity determination.
  • The findings support the development of more efficient biotechnological processes involving amylases.