Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD44 is a cytotoxic triggering molecule on human polymorphonuclear cells

F Pericle1, G Sconocchia, J A Titus

  • 1Experimental Immunology Branch, National Cancer Institute, Bethesda, MD 20892, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|November 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pre-transplant persistence of minimal residual disease does not contraindicate allogeneic stem cell transplantation for adult patients with acute myeloid leukemia.

Bone marrow transplantation·2016
Same author

Resolved-Sideband Laser Cooling in a Penning Trap.

Physical review letters·2016
Same author

SGK-1 protects kidney cells against apoptosis induced by ceramide and TNF-α.

Cell death & disease·2015
Same author

Minimal residual disease negativity in elderly patients with acute myeloid leukemia may indicate different postremission strategies than in younger patients.

Annals of hematology·2015
Same author

Inhibition of JAK3 with a novel, selective and orally active small molecule induces therapeutic response in T-cell malignancies.

Leukemia·2013
Same author

Fast shuttling of ions in a scalable Penning trap array.

The Review of scientific instruments·2010

CD44 triggers polymorphonuclear cells (PMN) to kill tumor cells. This interaction, especially with hyaluronic acid, suggests CD44

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Medicine

Background:

  • Polymorphonuclear cells (PMN) express high levels of CD44.
  • CD44 is a cell surface glycoprotein involved in cell adhesion and signaling.

Purpose of the Study:

  • To investigate the role of CD44 as a cytotoxic triggering molecule on PMN.
  • To explore the mechanism by which CD44 mediates tumor cell lysis by PMN.

Main Methods:

  • Flow cytometry (FACS) and immunoprecipitation to analyze CD44 expression on PMN.
  • Bispecific antibody-mediated assays to induce and assess PMN cytotoxicity against tumor cells.
  • Inhibition studies using anti-CD44 antibodies and hyaluronic acid (HA) to confirm CD44's role.

Main Results:

Related Experiment Videos

  • CD44 on PMN can trigger cytotoxic activity against tumor cells when cross-linked with bispecific antibodies.
  • PMN-mediated lysis of tumor cells by CD44 engagement does not require direct cell-cell contact and can affect bystander cells.
  • Target cell lysis is induced when cells are coated with hyaluronic acid, the ligand for CD44, and this is inhibited by anti-CD44 antibodies.

Conclusions:

  • CD44 acts as a cytotoxic triggering molecule on PMN.
  • The interaction of hyaluronic acid with CD44 on neutrophils may initiate cytotoxic or inflammatory responses in vivo, particularly in tumor microenvironments or areas with abundant extracellular matrix.