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Related Experiment Videos

Reduction of extracellular dehydroascorbic acid by K562 cells

E Schweinzer1, Y Mao, P Krajnik

  • 1Department of Medical Chemistry, University of Vienna, Austria.

Cell Biochemistry and Function
|March 1, 1996
PubMed
Summary

K562 cells reduce dehydroascorbic acid (DHA) to ascorbate, indicating a novel pathway for vitamin C production. This cellular reduction is dependent on cell number and DHA concentration.

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Area of Science:

  • Biochemistry
  • Cell Biology

Background:

  • Dehydroascorbic acid (DHA) is the oxidized form of vitamin C.
  • Understanding vitamin C metabolism is crucial for cellular health.

Purpose of the Study:

  • To investigate the ability of K562 erythroleukaemic cells to reduce dehydroascorbic acid (DHA) to ascorbate.
  • To characterize the kinetics and influencing factors of this reduction process.

Main Methods:

  • Incubation of K562 cells with varying concentrations of DHA.
  • Measurement of extracellular ascorbate production.
  • Kinetic analysis (Km, Vmax) and assessment of inhibitor effects.

Main Results:

  • K562 cells efficiently produce ascorbate from DHA in a dose- and cell-dependent manner.

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  • The reduction exhibits both high-affinity (Km ~7 μM) and low-affinity components.
  • The process is temperature-dependent, stimulated by glucose, and inhibited by phloretin and sulfhydryl reagents.

    • Conclusions:

    • K562 cells possess a significant capacity for extracellular reduction of DHA to ascorbate.
    • This pathway is distinct from intracellular reduction and release of ascorbate.
    • The findings suggest a novel mechanism for vitamin C generation in these cells.