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Related Experiment Videos

Epithelioid sarcoma with an 18q aberration

H Iwasaki1, Y Ohjimi, M Ishiguro

  • 1Departments of Pathology, Fukuoka University School of Medicine, Japan.

Cancer Genetics and Cytogenetics
|October 1, 1996
PubMed
Summary

Epithelioid sarcoma, a soft-tissue tumor, showed a new chromosomal abnormality der(22)t(18;22)(q11;p11.2). Stored tumor cells remained viable for cytogenetic analysis, supporting a link to synovial sarcoma.

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Area of Science:

  • Oncology
  • Cytogenetics
  • Soft Tissue Pathology

Background:

  • Epithelioid sarcoma is a rare soft-tissue neoplasm with uncertain origins, characterized by epithelioid cells expressing both epithelial (keratin) and nonepithelial (vimentin) markers.
  • Immunohistochemistry confirms epithelioid morphology and antigen expression (keratin, EMA, vimentin) in these tumors.

Observation:

  • A novel cytogenetic abnormality, der(22)t(18;22)(q11;p11.2), was identified in an epithelioid sarcoma case.
  • Tumor cells cultured from fresh surgical material were cryopreserved for six years, maintaining their original morphology and immunophenotype upon recovery.
  • Chromosome analysis and FISH revealed a modal population of 61-67 chromosomes with the characteristic der(22)t(18;22)(q11;p11.2) translocation and other numerical/structural aberrations.

Findings:

Related Experiment Videos

  • The identified chromosomal breakpoint at 18q11 is shared with synovial sarcoma, suggesting a potential histogenetic relationship between these tumor types.
  • Cryopreservation of tumor cells proved effective for subsequent detailed morphologic and cytogenetic investigations.

Implications:

  • This study expands the understanding of epithelioid sarcoma cytogenetics, identifying a new characteristic abnormality.
  • The findings support a possible evolutionary or histogenetic link between epithelioid sarcoma and synovial sarcoma.
  • Cryopreservation of soft tissue tumor cells is validated as a valuable method for long-term research and diagnostic analysis.