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Related Experiment Videos

Auditory cortical neurons in vitro: initial pharmacological studies

K V Gopal1, G W Gross

  • 1Department of Speech and Hearing Sciences, University of North Texas, Denton 76203, USA.

Acta Oto-Laryngologica
|September 1, 1996
PubMed
Summary

Murine auditory cortex neurons in culture showed basic pharmacological responses, indicating retained histiotypic properties. These neural networks offer a platform for studying auditory cortex network dynamics.

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Area of Science:

  • Neuroscience
  • Developmental Neuroscience
  • Cellular Neuroscience

Background:

  • Dissociated embryonic murine auditory cortex tissue can form spontaneously active monolayer networks in vitro.
  • These cultured networks can be maintained for extended periods (up to 113 days in vitro).

Purpose of the Study:

  • To investigate whether neurons in these cultured networks retain their original histiotypic (tissue-specific) properties.
  • To assess the functional responses of these cultured neural networks to specific neuroactive substances.

Main Methods:

  • Cultured monolayer networks from murine auditory cortex were exposed to a sequence of four synaptically active substances: bicuculline, strychnine, NMDA, and GABA.
  • Spontaneous network activity was recorded using multi-electrode arrays before and during substance application.

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  • Analysis involved chart recordings of integrated burst data and detailed processing of digitized short data segments.

    • Main Results:

    • Bicuculline induced oscillatory activity, simplified bursting, and increased burst regularity.
    • Strychnine application altered burst patterns and increased amplitude, suggesting glycine receptor presence.
    • NMDA increased burst frequency but decreased regularity and coordination, while GABA completely inhibited bursting.

    Conclusions:

    • Cultured monolayer networks from the murine auditory cortex exhibit basic histiotypic pharmacological responses.
    • These findings suggest that such networks are viable platforms for studying auditory cortex network dynamics and neuronal function in vitro.