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Increase in circulating products of lipid peroxidation in smokers with IDDM
G Zoppini1, G Targher, T Monauni
1Division of Endocrinology and Metabolic Diseases, University of Verona, Italy.
Objective:
To measure plasma malondialdehyde (MDA) concentration, a product of lipid peroxidation, both in IDDM patients and in healthy control subjects and to examine whether smoking has a negative impact on the plasma MDA levels in diabetic patients.
Research Design And Methods:
Plasma total MDA concentration (as a thiobarbituric acid adduct by high-performance liquid chromatography) was measured in 56 young IDDM patients and in a group of 32 age-, sex-, BMI-, and smoking habit-matched healthy subjects.
Results:
Plasma MDA concentration in IDDM patients was significantly higher than that in healthy control subjects (mean +/- SE: 0.95 +/- 0.03 vs. 0.54 +/- 0.03 mumol/l; P < 0.0001). After stratification by smoking status, it was seen that diabetic smokers had values of age, BMI, serum lipids, blood pressure, metabolic control, and diabetes duration and its chronic complications superimposable on those of their nonsmoking counterparts. Nevertheless, plasma MDA concentration was significantly higher in IDDM patients who smoked than in IDDM patients who didn't smoke (1.03 +/- 0.4 vs. 0.87 +/- 0.03 mumol/l; P = 0.002), without any sex difference with regard to MDA levels.
Conclusions:
These data show an increase in circulating products of lipid peroxidation in young diabetic smokers, thus further supporting the clinical importance of discouraging the initiation of smoking as well as promoting its cessation in people with IDDM.
Insights
Plasma malondialdehyde (MDA) levels, a marker of lipid peroxidation, are higher in patients with insulin-dependent diabetes mellitus (IDDM). Smoking further elevates MDA in diabetic individuals, highlighting the need for smoking cessation.
Area of Science:
- Biochemistry
- Endocrinology
- Cardiovascular Research
Background:
- Lipid peroxidation is a significant process in diabetes.
- Malondialdehyde (MDA) is a key biomarker for lipid peroxidation.
- Smoking is a known risk factor for cardiovascular disease and may exacerbate oxidative stress.
Purpose of the Study:
- To quantify plasma MDA levels in young patients with insulin-dependent diabetes mellitus (IDDM) compared to healthy controls.
- To investigate the impact of smoking on plasma MDA concentrations in IDDM patients.
Main Methods:
- Plasma MDA concentration was measured using high-performance liquid chromatography (HPLC).
- The study included 56 young IDDM patients and 32 matched healthy control subjects.
- Participants were stratified by smoking status to analyze its effect.
Main Results:
- IDDM patients exhibited significantly higher plasma MDA levels than healthy controls (0.95 vs. 0.54 mumol/l).
- Diabetic smokers had markedly elevated MDA levels compared to non-smoking diabetic patients (1.03 vs. 0.87 mumol/l).
- No significant differences in diabetes duration, metabolic control, or complications were observed between smoking and non-smoking diabetic groups.
Conclusions:
- Elevated circulating lipid peroxidation products are evident in young IDDM patients, particularly smokers.
- These findings underscore the critical importance of smoking cessation and prevention in individuals with IDDM to mitigate oxidative stress.