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Expression of the beta 7 integrin by human endothelial cells
R I Brezinschek1, H P Brezinschek, A I Lazarovits
1Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas 75235-8577, USA.
The American Journal of Pathology
|November 1, 1996
Summary
Researchers found that human endothelial cells express beta 7 integrin, a molecule previously identified on leukocytes. This finding suggests a new role for beta 7 integrin in endothelial cell function and interactions.
Area of Science:
- Cell Biology
- Immunology
- Molecular Biology
Background:
- Integrin adhesion receptors are crucial for cell-cell and cell-extracellular matrix interactions.
- Beta 7 integrin (ITGB7) has been primarily identified in leukocyte subsets, mediating their adhesion and migration.
- The role of beta 7 integrin in non-hematopoietic cells, particularly endothelial cells, remains largely unexplored.
Purpose of the Study:
- To investigate the expression and potential function of beta 7 integrin in human endothelial cells.
- To determine if endothelial beta 7 integrin is similar to that found in leukocytes.
- To examine the regulation of beta 7 integrin expression on endothelial cells by inflammatory stimuli.
Main Methods:
- Analysis of complementary DNA (cDNA) to compare beta 7 integrin sequences.
- Cell surface expression analysis of beta 7 integrin on endothelial cells in vitro and in situ.
- Treatment of endothelial cells with pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-1 beta) to assess changes in beta 7 expression.
- Assessment of alpha-integrin chain expression in endothelial cells.
Main Results:
- Human endothelial cells express beta 7 integrin both in vitro and in situ.
- The cDNA sequence of endothelial beta 7 integrin is identical to that expressed by leukocytes.
- Pro-inflammatory cytokines significantly increase cell surface expression of beta 7 integrin on endothelial cells.
- Endothelial cells express alpha 4 integrin but not alpha E integrin, suggesting potential complex formation with beta 7.
Conclusions:
- Endothelial cells express beta 7 integrin, challenging the previous understanding of its restricted expression.
- The expression of beta 7 integrin on endothelial cells is inducible by inflammatory signals.
- Beta 7 integrin, likely complexed with alpha 4 integrin, may play a significant role in endothelial cell function and phenotype maintenance.
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