Lactate dehydrogenase isoenzymes of human erythrocyte membranes

Human erythrocyte membranes and cytosol were fractionated in detergent-containing polyacrylamide gels and stained for lactate dehydrogenase activity. A highly significant difference in the distribution of three isoenzymes of lactate dehydrogenase (LD-1, LD-2 and LD-3) between membranes and cytosol was found, a striking feature being the poor binding of LD-3 to the membrane fraction.